Journal: Reproductive Sciences
Article Title: The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles
doi: 10.1007/s43032-021-00691-3
Figure Lengend Snippet: Protein expression in CCs and MGCs. Primary antibodies: mouse anti–human ABCA1 polyclonal antibody (Abcam; diluted 1:3000); rabbit anti–human LDLR polyclonal antibody (PTG; diluted 1:1000); rabbit anti–human SCARB1 polyclonal antibody (Abcam; diluted 1:1000); rabbit anti–human SULT2B1 polyclonal antibody (Abclonal; diluted 1:1000); rabbit anti–human CYP19A1 polyclonal antibody (Abclonal; diluted 1:1000); rabbit anti–human CRLS1 polyclonal antibody (PTG; diluted 1:1000); mouse anti–human LPCAT3 polyclonal antibody (Abcam; diluted 1:1000); rabbit anti–human PLA2G4A polyclonal antibody (Abclonal; diluted 1:1000). Secondary antibodies: goat anti-mouse IgG conjugated with horseradish peroxidase (HRP) diluted 1:3000 in TTBS for ABCA1 and LPCAT3 or goat anti–rabbit IgG conjugated with HRP diluted 1:3000 in TTBS for LDLR, SCARB1, SULT2B1, CYP19A1, CRLS1, and PLA2G4A. The expression of mouse anti–human ACTIN polyclonal antibody (Servicebio; diluted 1:3000) was used as an internal reference. A shows the protein expression of ABCA1, LDLR, SCARB1, SULT2B1, and CYP19A1; B shows the protein expression of CRLS1, LPCAT3, and PLA2G4A
Article Snippet: Primary antibodies: mouse anti–human ABCA1 polyclonal antibody (Abcam; diluted 1:3000); rabbit anti–human LDLR polyclonal antibody (PTG; diluted 1:1000); rabbit anti–human SCARB1 polyclonal antibody (Abcam; diluted 1:1000); rabbit anti–human SULT2B1 polyclonal antibody (Abclonal; diluted 1:1000); rabbit anti–human CYP19A1 polyclonal antibody (Abclonal; diluted 1:1000); rabbit anti–human CRLS1 polyclonal antibody (PTG; diluted 1:1000); mouse anti–human LPCAT3 polyclonal antibody (Abcam; diluted 1:1000); rabbit anti–human PLA2G4A polyclonal antibody (Abclonal; diluted 1:1000).
Techniques: Expressing